Chemosensors and biosensors based on polyelectrolyte microcapsules containing fluorescent dyes and enzymes.

@article{Kazakova2013ChemosensorsAB,
  title={Chemosensors and biosensors based on polyelectrolyte microcapsules containing fluorescent dyes and enzymes.},
  author={Lyubov I Kazakova and Lyudmila I Shabarchina and Salzitsa Anastasova and Anton M. Pavlov and Pankaj Vadgama and Andre G Skirtach and Gleb B. Sukhorukov},
  journal={Analytical and bioanalytical chemistry},
  year={2013},
  volume={405 5},
  pages={1559-68}
}
The concept of enzyme-assisted substrate sensing based on use of fluorescent markers to detect the products of enzymatic reaction has been investigated by fabrication of micron-scale polyelectrolyte capsules containing enzymes and dyes in one entity. Microcapsules approximately 5 μm in size entrap glucose oxidase or lactate oxidase, with peroxidase, together with the corresponding markers Tris(4,7-diphenyl-1,10-phenanthroline)ruthenium(II) dichloride (Ru(dpp)) complex and dihydrorhodamine 123… CONTINUE READING