Chemoenzymatic synthesis and in situ application of S-adenosyl-L-methionine analogs.

@article{Thomsen2013ChemoenzymaticSA,
  title={Chemoenzymatic synthesis and in situ application of S-adenosyl-L-methionine analogs.},
  author={Marie Thomsen and Stine Byskov Vogensen and Jens Buchardt and Michael D. Burkart and Rasmus Pr{\ae}torius Clausen},
  journal={Organic & biomolecular chemistry},
  year={2013},
  volume={11 43},
  pages={7606-10}
}
Analogs of S-adenosyl-L-methionine (SAM) are increasingly applied to the methyltransferase (MT) catalysed modification of biomolecules including proteins, nucleic acids, and small molecules. However, SAM and its analogs suffer from an inherent instability, and their chemical synthesis is challenged by low yields and difficulties in stereoisomer isolation and inhibition. Here we report the chemoenzymatic synthesis of a series of SAM analogs using wild-type (wt) and point mutants of two recently… CONTINUE READING