Chemoenzymatic Semisynthesis of Proteins.

@article{Thompson2019ChemoenzymaticSO,
  title={Chemoenzymatic Semisynthesis of Proteins.},
  author={Robert E Thompson and Tom W. Muir},
  journal={Chemical reviews},
  year={2019}
}
Protein semisynthesis-defined herein as the assembly of a protein from a combination of synthetic and recombinant fragments-is a burgeoning field of chemical biology that has impacted many areas in the life sciences. In this review, we provide a comprehensive survey of this area. We begin by discussing the various chemical and enzymatic methods now available for the manufacture of custom proteins containing noncoded elements. This section begins with a discussion of methods that are more… Expand
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  • Biology
  • 2021
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A protein semi-synthesis strategy via salicylaldehyde ester-mediated chemical ligations (Ser/Thr ligation and Cys/Pen ligation) has enabled us to generate Lys (2/6/7/11) tetra-acetylated HMGB1 (high-mobility group box 1) protein, a 25 kDa proinflammatory protein, in high purity. Expand
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