Chemo-enzymatic synthesis of polyhydroxyalkanoate by an improved two-phase reaction system (TPRS).

@article{Han2009ChemoenzymaticSO,
  title={Chemo-enzymatic synthesis of polyhydroxyalkanoate by an improved two-phase reaction system (TPRS).},
  author={Xuerong Han and Yasuharu Satoh and Kenji Tajima and Tatsuo Matsushima and Masanobu Munekata},
  journal={Journal of bioscience and bioengineering},
  year={2009},
  volume={108 6},
  pages={
          517-23
        }
}
In our previous paper, we synthesized poly-3-hydroxybutyrate [P(3HB)] by using the water-organic solvent two-phase reaction system (TPRS), in which thiophenyl (R)-3-hydroxybutyrate [(R)-3HBTP] was used as a precursor of 3HBCoA. We have developed an improved TPRS for the chemo-enzymatic synthesis of polyhydroxyalkanoate (PHA). In this method, acetyl thioester of ethyl thioglycolate (AcETG) was used as a precursor of acetylCoA (AcCoA), which was a donor of CoA. The AcCoA was formed by the ester… CONTINUE READING
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Chemoenzymatic synthesis of poly(3-hydroxybutyrate) in a water-organic solvent two-phase system

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