• Chemistry, Medicine
  • Published in
    Organic & biomolecular…
    2005
  • DOI:10.1039/b409583a

Chemo-enzymatic synthesis of 4-methylumbelliferyl beta-(1-->4)-D-xylooligosides: new substrates for beta-D-xylanase assays.

@article{Eneyskaya2005ChemoenzymaticSO,
  title={Chemo-enzymatic synthesis of 4-methylumbelliferyl beta-(1-->4)-D-xylooligosides: new substrates for beta-D-xylanase assays.},
  author={Elena V. Eneyskaya and Dina R. Ivanen and Konstantin Shabalin and Anna A Kulminskaya and Leon Vladimirovich Backinowsky and Harry Brumer and Kirill N. Neustroev},
  journal={Organic & biomolecular chemistry},
  year={2005},
  volume={3 1},
  pages={
          146-51
        }
}
Transglycosylation catalyzed by a beta-D-xylosidase from Aspergillus sp. was used to synthesize a set of 4-methylumbelliferyl (MU) beta-1-->4-D-xylooligosides having the common structure [beta-D-Xyl-(1-->4)]2-5-beta-D-Xyl-MU. MU xylobioside synthesized chemically by the condensation of protected MU beta-D-xylopyranoside with ethyl 2,3,4-tri-O-acetyl-1-thio-beta-D-xylopyranoside was used as a substrate for transglycosylation with the beta-D-xylosidase from Aspergillus sp. to produce higher MU… CONTINUE READING