Chemistry in living systems

  title={Chemistry in living systems},
  author={Jennifer A. Prescher and Carolyn R. Bertozzi},
  journal={Nature Chemical Biology},
Dissecting complex cellular processes requires the ability to track biomolecules as they function within their native habitat. Although genetically encoded tags such as GFP are widely used to monitor discrete proteins, they can cause significant perturbations to a protein's structure and have no direct extension to other classes of biomolecules such as glycans, lipids, nucleic acids and secondary metabolites. In recent years, an alternative tool for tagging biomolecules has emerged from the… 

Bioorthogonal chemistry: a covalent strategy for the study of biological systems

The recent development of bioorthogonal reactions and their applications in various biological systems are reviewed, with a detailed discussion about the development of the tetrazole based, photoinducible 1,3-dipolar cycloaddition reaction.

Introducing bioorthogonal functionalities into proteins in living cells.

A facile pyrrolysine-based system, which might potentially become the "one-stop shop" for protein modification in both prokaryotic and eukaryotic cells, has recently emerged and may provide more precise protein labeling than GFP tagging.

Chemical Tools for Imaging Glycans in Living Systems

A two-step method to equip glycans with reporter tags for isolation and visualization from living systems known as the chemical reporter strategy is developed.

Chemical reporters for biological discovery.

How chemical reporters in conjunction with bioorthogonal labeling methods can be used to image and retrieve nucleic acids, proteins, glycans, lipids and other metabolites in vitro, in cells as well as in whole organisms is reviewed.

Fluorescent analogs of biomolecular building blocks: design, properties, and applications.

This article focuses on designing fluorescent probes for the four major families of macromolecular building blocks discussed above, and discusses emissive carbohydrate derivatives, followed byEmissive amino acids, the building blocks of nucleic acids.

Copper-free click chemistry for dynamic in vivo imaging

A Cu-free variant of click chemistry that can label biomolecules rapidly and selectively in living systems, overcoming the intrinsic toxicity of the canonical Cu-catalyzed reaction is reported.

Chemical strategies for tagging and imaging the proteome.

  • K. Beatty
  • Biology, Chemistry
    Molecular bioSystems
  • 2011
This review provides an overview of the methods which have been optimized to tag and fluorophore-label biomolecules for imaging subsets of the proteome in bacterial and mammalian cells.

Proteins: Chemistry and Chemical Reactivity

An introduction to the chemical reactivity of the amino acid side chains is provided, with an emphasis on the selectivity that can be achieved using a particular reactive strategy.

Bioorthogonal chemistry: fishing for selectivity in a sea of functionality.

The bioorthogonal chemical reactions developed to date are described and how they can be used to study biomolecules.

Azide-based bioorthogonal chemistry: Reactions and its advances in cellular and biomolecular imaging

The development of bioorthogonal reporters and their capability of being built into biomolecules in vivo have been extensively applied in cellular imaging and this review discusses the milestones of azide-based bioorthogsonal reactions.



Bioorthogonal organic chemistry in living cells: novel strategies for labeling biomolecules.

With the advances in biocompatible synthetic organic chemistry, a whole new field of opportunity has opened up, affording high diversity in the nature of the label as well as a choice of ligation reactions.

Protein semi-synthesis in living cells.

  • I. GiriatT. Muir
  • Biology, Chemistry
    Journal of the American Chemical Society
  • 2003
This work describes an approach that allows ligation of synthetic molecules to target proteins in an intracellular environment through a peptide bond, specific and applicable to cytosolic and integral membrane proteins.

Activity-based protein profiling in vivo using a copper(i)-catalyzed azide-alkyne [3 + 2] cycloaddition.

It is shown that several enzymes can be labeled in an activity-based manner both in vitro and in vivo by an azido-sulfonate ester probe and that these labeling events can be detected in whole proteomes by copper-catalyzed ligation with a rhodamine-alkyne reagent.

A Fusion of Disciplines: Chemical Approaches to Exploit Fusion Proteins for Functional Genomics

A new class of tags that are not limited to their genetically encoded function but rather serves as general acceptors for synthetic mols.

Cell surface engineering by a modified Staudinger reaction.

A chemical transformation that permits the selective formation of covalent adducts among richly functionalized biopolymers within a cellular context is presented and should permit its execution within a cell's interior, offering new possibilities for probing intracellular interactions.

Reversible site-selective labeling of membrane proteins in live cells

The ionotropic 5HT3 serotonin receptor is investigated by fluorescence measurements to characterize in vivo the probe-receptor interactions, yielding information on structure and plasma membrane distribution of the receptor.

Engineering chemical reactivity on cell surfaces through oligosaccharide biosynthesis.

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