Chemiluminescence assay of N-acetyl-beta-D-glucosaminidase in urine using o-aminophthalylhydrazido-N-acetyl-beta-D-glucosaminide.

  title={Chemiluminescence assay of N-acetyl-beta-D-glucosaminidase in urine using o-aminophthalylhydrazido-N-acetyl-beta-D-glucosaminide.},
  author={Kaname Sasamoto and R Zenko and Kohji Ueno and Yasufumi Ohkura},
  journal={Chemical & pharmaceutical bulletin},
  volume={39 5},
The chemiluminescence assay of urinary N-acetyl-beta-D-glucosaminidase using o-aminophthalylhydrazido-N-acetyl-beta-D-glucosaminide as the substrate was examined. Under optimized conditions, a linear chemiluminescence response to the standard N-acetyl-beta-D-glucosaminidase (NAGase) of different concentrations was observed with a correlation coefficient of 0.999 over the range of 0.3 to 20 I.U./l. The present method was compared with the prevalent method using p-nitrophenyl-N-acetyl-beta-D… CONTINUE READING