Chemiluminescence-HPLC assay of phosphatidylcholine hydroperoxide generated by ischemia-reperfusion in the liver of rats.

@article{Takayama1992ChemiluminescenceHPLCAO,
  title={Chemiluminescence-HPLC assay of phosphatidylcholine hydroperoxide generated by ischemia-reperfusion in the liver of rats.},
  author={Fusako Takayama and Toru Egashira and Yoshihisa Kudo and Yasumitsu Yamanaka},
  journal={Biochemical pharmacology},
  year={1992},
  volume={44 12},
  pages={2412-4}
}
To determine cellular damage due to "oxidative stress", we developed a sensitive and specific quantitative assay for phosphatidylcholine hydroperoxide (PCOOH) by coupling HPLC with detection of chemiluminescence (CL). The qualitative and quantitative detection limits of PCOOH by this assay were 0.5 and 2 pmol (based on active oxygen from hydroperoxide). Using this CL-HPLC method, we determined PCOOH levels caused by ischemia-reperfusion in rat livers. The PCOOH levels in livers of control, sham… CONTINUE READING