Chemiluminescence Determination of D-amino Acid Based on Immobilized Enzymes

@inproceedings{Man1996ChemiluminescenceDO,
  title={Chemiluminescence Determination of D-amino Acid Based on Immobilized Enzymes},
  author={Feng Man},
  year={1996}
}
D-amino acid oxidase and HRP were separately immobilized on chitosan adsorbed on the silica gels to develop a chemiluminescent method,which was combined with two oxidation reactions of D-amino acid producing H2O2 catalyzed by D-amino acid oxidase and of Luminol-H2O2 generating CL catalyzed by HRP together,also was stopped──flow technique used in this system. It has a good selectivity and sensitivity. Under optimized conditions,the detection limit is 1.8 ×10 ̄(-5)g/mL,the liner range of D-amino… CONTINUE READING