Chemically defined media and the culture of mammalian preimplantation embryos: historical perspective and current issues.

@article{Summers2003ChemicallyDM,
  title={Chemically defined media and the culture of mammalian preimplantation embryos: historical perspective and current issues.},
  author={Michael C. Summers and John D. Biggers},
  journal={Human reproduction update},
  year={2003},
  volume={9 6},
  pages={
          557-82
        }
}
Considerable advances in media development for the culture of preimplantation mammalian embryos have been made since mouse embryos were first cultured and successfully transferred to foster mothers. The purpose of this review is to detail the history of the development of chemically defined media for the culture of preimplantation embryos. Two approaches have been used to determine the composition of chemically defined media: the 'back-to-nature' approach and 'let the embryo choose' or… Expand
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References

SHOWING 1-10 OF 427 REFERENCES
Reflections on the culture of the preimplantation embryo.
  • J. Biggers
  • Biology, Medicine
  • The International journal of developmental biology
  • 1998
TLDR
It is concluded that even the best available media inevitably cause imbalances in the environment in which the embryos are forced to develop, because they consist of only a small subset of the compounds present in the natural environments. Expand
Fundamentals of the Design of Culture Media that Support Human Preimplantation Development
Human preimplantation embryos were first cultured from the zygote to the sixteen cell stage by Edwards et al. (1970). Later, human zygotes were cultured in vitro to the blastocyst stage (Steptoe etExpand
Culture of preimplantation embryos: facts and artifacts.
  • B. Bavister
  • Biology, Medicine
  • Human reproduction update
  • 1995
TLDR
In this review, comparative information on epigenetic regulation of embryo development is discussed, including information on energy substrate and amino acid preferences of embryos and improvements in the design of culture media are discussed, so that higher yields and increased viability of embryos are achieved. Expand
Biological Factors in Culture Media Affecting in Vitro Fertilization, Preimplantation Embryo Development, and Implantation
TLDR
Water is the basis of all culture media, and ultrapure water should be employed, and a fixed nitrogen source is essential for implantation embryo development whether this is provided by amino acids, albumin, or serum. Expand
Differential preimplantation development of rhesus monkey embryos in serum‐supplemented media
TLDR
Results indicate that a simple medium, KSOM/AA, in the presence of serum, supports the development of rhesus monkey embryos at high efficiency and at a faster rate than that observed for embryos cultured in the complex medium, CMRL‐1066. Expand
Development of Viable Mammalian Embryos In Vitro : Evolution of Sequential Media
TLDR
This chapter focuses on the utility of sequential media in optimizing mammalian embryo development in culture and the fact that the sequential culture media, G1 and G2, can support the development of viable blastocysts of the primate, rodent, and ruminant, despite the species differences in embryo physiology. Expand
Differential growth of the mouse preimplantation embryo in chemically defined media.
TLDR
The total cell number of KSOM-cultured blastocysts indicates that 5-6 cell divisions are possible when embryos are cultured for 96 h from pronuclear zygotes, a significant improvement in performance over that of other defined media for the culture of zygote to blastocyst stage. Expand
Genetic regulation of preimplantation embryo survival.
TLDR
Embryo imaging is an exciting new field and the data garnered by these new imaging modalities may in due course significantly enhance and complement the genetic data discussed in this chapter. Expand
Effects of the in vitro chemical environment during early embryogenesis on subsequent development.
  • D. Rieger
  • Biology, Medicine
  • Archives of toxicology. Supplement. = Archiv fur Toxikologie. Supplement
  • 1998
TLDR
The production of a morphologically normal embryo is no guarantee that fetal development and post-natal life will be normal, and this is of immediate concern in human reproductive medicine due to the increasing use of sperm injection for fertilization, and the emergence of in-vitro oocyte maturation. Expand
Successful Development and Birth of Mice cultivated in vitro as Early Embryos
TLDR
The first successful attempt to combine the techniques of embryo culture and embryo transplantation was briefly reported by Adams1, and normal embryos in the uterus of the recipient female, presumably developed from the cultured embryos. Expand
...
1
2
3
4
5
...