Chemically charging the pore constriction opens the mechanosensitive channel MscL.

@article{Yoshimura2001ChemicallyCT,
  title={Chemically charging the pore constriction opens the mechanosensitive channel MscL.},
  author={Kotaro Yoshimura and Ann Finney Batiza and Ching Kung},
  journal={Biophysical journal},
  year={2001},
  volume={80 5},
  pages={2198-206}
}
MscL is a bacterial mechanosensitive channel that protects the cell from osmotic downshock. We have previously shown that substitution of a residue that resides within the channel pore constriction, MscL's Gly-22, with all other 19 amino acids affects channel gating according to the hydrophobicity of the substitution (). Here, we first make a mild substitution, G22C, and then attach methanethiosulfonate (MTS) reagents to the cysteine under patch clamp. Binding MTS reagents that are positively… CONTINUE READING

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