Chemical transformation of yeast.

Abstract

Transformation of chemically competent yeast cells is a method for introducing exogenous DNA into living cells. Typically, the DNA is either a plasmid carrying an autonomous replication sequence that allows for propagation or a linear piece of DNA to be integrated into the genome. The DNA usually also carries a marker that allows for selection of… (More)
DOI: 10.1016/B978-0-12-418687-3.00026-4

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