Chemical synthesis of biologically active tat trans-activating protein of human immunodeficiency virus type 1.

@article{Chun1990ChemicalSO,
  title={Chemical synthesis of biologically active tat trans-activating protein of human immunodeficiency virus type 1.},
  author={Raymond Y. T. Chun and Charles G. Glabe and Hung Fan},
  journal={Journal of virology},
  year={1990},
  volume={64 6},
  pages={
          3074-7
        }
}
Full-length (86-residue) polypeptide corresponding to the human immunodeficiency virus type 1 tat trans-activating protein was chemically synthesized on a semiautomated apparatus, using an Fmoc amino acid continuous-flow strategy. The bulk material was relatively homogeneous, as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and isoelectric focusing, and it showed trans-activating activity when scrape loaded into cells containing a human immunodeficiency virus long terminal… CONTINUE READING