Chemical synthesis and expression of a cassette adapted ubiquitin gene.

@article{Ecker1987ChemicalSA,
  title={Chemical synthesis and expression of a cassette adapted ubiquitin gene.},
  author={Dave J Ecker and Mazhar I. Khan and Jon K Marsh and Tauseef R. Butt and Stanley T. Crooke},
  journal={The Journal of biological chemistry},
  year={1987},
  volume={262 8},
  pages={3524-7}
}
A gene encoding the yeast ubiquitin was chemically synthesized and expressed in yeast under regulatory control of the copper metallothionein (CUP1) promoter. The gene was assembled in a one-step ligation reaction from eight oligonucleotide fragments ranging in length from 50 to 64 nucleotides. To facilitate mutagenesis and gene fusion studies, eight unique… CONTINUE READING