Chemical synthesis and cloning of a poly(arginine)-coding gene fragment designed to aid polypeptide purification.

@article{Smith1984ChemicalSA,
  title={Chemical synthesis and cloning of a poly(arginine)-coding gene fragment designed to aid polypeptide purification.},
  author={J. C. Smith and Roger Derbyshire and Edwin Cook and L Dunthorne and Jo Viney and Stephen J. Brewer and Helmut M. Sassenfeld and Lippman Bell},
  journal={Gene},
  year={1984},
  volume={32 3},
  pages={321-7}
}
A 43-bp DNA duplex coding for poly(arginine) [poly(arg)] has been synthesised by modified phosphotriester procedures. It has been inserted into the Bg/II and BamHI restriction sites of a cloned synthetic beta-urogastrone (Uro) gene, under the control of the trp promoter. Subsequent induction with 3 beta-indole acrylic acid produces beta-Uro with a C-terminal poly(arg) fusion. The raised isoelectric point of this polypeptide fusion facilitates rapid purification by cation exchange chromatography… CONTINUE READING

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