Chemical subdomains within the kinetochore domain of isolated CHO mitotic chromosomes

  title={Chemical subdomains within the kinetochore domain of isolated CHO mitotic chromosomes},
  author={Linda Wordeman and Eric R Steuer and Michael P Sheetz and Timothy J Mitchison},
  journal={The Journal of Cell Biology},
  pages={285 - 294}
We have used indirect immunofluorescence in combination with correlative EM to subdivide the mammalian kinetochore into two domains based on the localization of specific antigens. We demonstrate here that the fibrous corona on the distal face of the kinetochore plate contains tubulin (previously shown by Mitchison, T. J., and M. W. Kirschner. 1985. J. Cell Biol. 101:755-765) and the minus end-directed, ATP-dependent microtubule motor protein, dynein; whereas a 50-kD CREST antigen is located… CONTINUE READING


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