Chemical subdomains within the kinetochore domain of isolated CHO mitotic chromosomes

@article{Wordeman1991ChemicalSW,
  title={Chemical subdomains within the kinetochore domain of isolated CHO mitotic chromosomes},
  author={Linda Wordeman and Eric R Steuer and Michael P Sheetz and Timothy J Mitchison},
  journal={The Journal of Cell Biology},
  year={1991},
  volume={114},
  pages={285 - 294}
}
We have used indirect immunofluorescence in combination with correlative EM to subdivide the mammalian kinetochore into two domains based on the localization of specific antigens. We demonstrate here that the fibrous corona on the distal face of the kinetochore plate contains tubulin (previously shown by Mitchison, T. J., and M. W. Kirschner. 1985. J. Cell Biol. 101:755-765) and the minus end-directed, ATP-dependent microtubule motor protein, dynein; whereas a 50-kD CREST antigen is located… CONTINUE READING

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References

Publications referenced by this paper.
Showing 1-10 of 14 references

MAP 1 C is a microubuleactivated ATPase which translocates micrombules in vitro and has dyneinlike properties

  • B. M. Paschal, H. S. Shpetner
  • J . Cell Biol .
  • 1987

Mitosi s in Oedegonium : spindle microfilaments and the origin of the kinetochore fiber

  • M. J. Schlibler
  • J . Cell Biol .
  • 1980

Chromosome studies in chionographis liliaceae

  • Tanaka, N . Tanaka
  • Cytologia
  • 1979

Effect of N-ethylmaleimide and of heat

  • Blum, J J., A . Hayes
  • 1974

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