Chemical screens against a reconstituted multiprotein complex: myricetin blocks DnaJ regulation of DnaK through an allosteric mechanism.

@article{Chang2011ChemicalSA,
  title={Chemical screens against a reconstituted multiprotein complex: myricetin blocks DnaJ regulation of DnaK through an allosteric mechanism.},
  author={Lyra Chang and Yoshinari Miyata and Peter M.-U. Ung and Eric B. Bertelsen and Thomas J. McQuade and Heather A. Carlson and Erik R P Zuiderweg and Jason E. Gestwicki},
  journal={Chemistry & biology},
  year={2011},
  volume={18 2},
  pages={210-21}
}
DnaK is a molecular chaperone responsible for multiple aspects of bacterial proteostasis. The intrinsically slow ATPase activity of DnaK is stimulated by its co-chaperone, DnaJ, and these proteins often work in concert. To identify inhibitors we screened plant-derived extracts against a reconstituted mixture of DnaK and DnaJ. This approach resulted in the identification of flavonoids, including myricetin, which inhibited activity by up to 75%. Interestingly, myricetin prevented DnaJ-mediated… CONTINUE READING

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