Chemical pulldown reveals dynamic pseudouridylation of the mammalian transcriptome.

@article{Li2015ChemicalPR,
  title={Chemical pulldown reveals dynamic pseudouridylation of the mammalian transcriptome.},
  author={X Li and Ping Zhu and Shiqing Ma and Jinghui Song and Jinyi Bai and Fangfang Sun and Chengqi Yi},
  journal={Nature chemical biology},
  year={2015},
  volume={11 8},
  pages={592-7}
}
Pseudouridine (Ψ) is the most abundant post-transcriptional RNA modification, yet little is known about its prevalence, mechanism and function in mRNA. Here, we performed quantitative MS analysis and show that Ψ is much more prevalent (Ψ/U ratio ∼0.2-0.6%) in mammalian mRNA than previously believed. We developed N3-CMC-enriched pseudouridine sequencing (CeU-Seq), a selective chemical labeling and pulldown method, to identify 2,084 Ψ sites within 1,929 human transcripts, of which four (in… CONTINUE READING
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