Chemical proteomics with sulfonyl fluoride probes reveals selective labeling of functional tyrosines in glutathione transferases.

@article{Gu2013ChemicalPW,
  title={Chemical proteomics with sulfonyl fluoride probes reveals selective labeling of functional tyrosines in glutathione transferases.},
  author={C. Gu and D. A. Shannon and T. Colby and Zhemin Wang and M. Shabab and S. Kumari and Joji Grace Villamor and Christopher J. McLaughlin and E. Weerapana and M. Kaiser and B. Cravatt and R. V. D. van der Hoorn},
  journal={Chemistry \& biology},
  year={2013},
  volume={20 4},
  pages={
          541-8
        }
}
Chemical probes have great potential for identifying functional residues in proteins in crude proteomes. Here we studied labeling sites of chemical probes based on sulfonyl fluorides (SFs) on plant and animal proteomes. Besides serine proteases and many other proteins, SF-based probes label Tyr residues in glutathione transferases (GSTs). The labeled GSTs represent four different GST classes that share less than 30% sequence identity. The targeted Tyr residues are located at similar positions… Expand

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