Chemical probes for higher-order structure in RNA.

  title={Chemical probes for higher-order structure in RNA.},
  author={Debra A. Peattie and Walter Gilbert},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={77 8},
Three chemical reactions can probe the secondary and tertiary interactions of RNA molecules in solution. Dimethyl sulfate monitors the N-7 of guanosines and senses tertiary interactions there, diethyl pyrocarbonate detects stacking of adenosines, and an alternate dimethyl sulfate reaction examines the N-3 of cytidines and thus probes base pairing. The reactions work between 0 degrees C and 90 degrees C and at pH 4.5--8.5 in a variety of buffers. As an example we follow the progressive… CONTINUE READING
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