Chemical modification of spinach plastocyanin: separation and characterization of four different forms.

Abstract

Spinach plastocyanin has been chemically modified by using a water-soluble carbodiimide to form an amide bond between a protein carboxyl group and one amino group of ethylenediamine. This reaction produced four distinct species of chemically modified plastocyanin which could be separated either by polyacrylamide gel electrophoresis (in the absence of sodium… (More)

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