Chemical modification and site-directed mutagenesis of methionine residues in recombinant human granulocyte colony-stimulating factor: effect on stability and biological activity.

@article{Lu1999ChemicalMA,
  title={Chemical modification and site-directed mutagenesis of methionine residues in recombinant human granulocyte colony-stimulating factor: effect on stability and biological activity.},
  author={Hsieng Sen Lu and P R Fausset and Linda Owers Narhi and Thomas P. Horan and Kunihiro Shinagawa and Grant T. Shimamoto and Thomas C. Boone},
  journal={Archives of biochemistry and biophysics},
  year={1999},
  volume={362 1},
  pages={1-11}
}
Chemical modification and mutagenesis of methionines in recombinant human granulocyte colony-stimulating factor (G-CSF) were investigated. Selective oxidation of G-CSF by H2O2 and t-butyl hydroperoxide leads to generation of different oxidized forms. Four modified forms were isolated and shown to contain 1 to 4 oxidized methionyl residues. All methionines in G-CSF are reactive, with reaction kinetics following the order of Met1>Met138>Met127>>>Met122. H2O2 oxidation of Met122 is relatively slow… CONTINUE READING

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European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V • 2011

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