Chemical modification and irreversible inhibition of striatal A2a adenosine receptors.

@article{Jacobson1992ChemicalMA,
  title={Chemical modification and irreversible inhibition of striatal A2a adenosine receptors.},
  author={Kenneth A. Jacobson and Gary L. Stiles and X D Ji},
  journal={Molecular pharmacology},
  year={1992},
  volume={42 1},
  pages={123-33}
}
The ligand recognition site of A2a-adenosine receptors in rabbit striatal membranes was probed using non-site-directed labeling reagents and specific affinity labels. Exposure of membranes to diethylpyrocarbonate at a concentration of 2.5 mM, followed by washing, was found to inhibit the binding of [3H]CGS 21680 and [3H]xanthine amine congener to A2a receptors, by 86 and 30%, respectively. Protection from diethylpyrocarbonate inactivation by an adenosine receptor agonist, 5'-N… CONTINUE READING