Chemical mechanism of saccharopine dehydrogenase (NAD+, L-lysine-forming) as deduced from initial rate pH studies.

@article{Fujioka1984ChemicalMO,
  title={Chemical mechanism of saccharopine dehydrogenase (NAD+, L-lysine-forming) as deduced from initial rate pH studies.},
  author={Motoji Fujioka},
  journal={Archives of biochemistry and biophysics},
  year={1984},
  volume={230 2},
  pages={553-9}
}
  • Motoji Fujioka
  • Published 1984 in Archives of biochemistry and biophysics
The variation of kinetic parameters with pH has been determined so as to gain insight into the chemical mechanism of the saccharopine dehydrogenase (NAD+,L-lysine-forming)-catalyzed reaction. In the direction of reductive condensation of lysine and alpha-ketoglutarate (reverse reaction), the V/K profile for lysine shows a group with a pK of 6.3 must be unprotonated and a group with a pK of 8.0 must be protonated for activity. Similar pK's are obtained in the pKi profile for ornithine, which… CONTINUE READING

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