Chemical ionization mass spectrometry of complex molecules—VI: Peptides†

  title={Chemical ionization mass spectrometry of complex molecules—VI: Peptides†},
  author={A. Kiryushkin and H. Fales and T. Axenrod and E. J. Gilbert and G. Milne},
  journal={Journal of Mass Spectrometry},
The chemical ionization mass spectra of a series of simple peptides containing six or fewer amino acids have been studied. Using methane as the reactant gas we found cleavage of the peptide bond occurs in two ways, yielding either the acyl carbonium ion or the complementary ammonium ion. The observation of both types of fragments permits the determination of the amino acid sequence of the peptide. The ammonium ions provide an additional sequence determining route compared to that available from… Expand
52 Citations
Peptide sequencing: the utility of chemical ionization mass spectrometry.
In prospect, isobutane chemical ionization mass spectrometry seems to be a useful technique for peptide sequence determination, and may have advantages in some cases. Expand
Chemical ionzation mass spectra of amino acids and derivatives. Occurrence and fragmentation of ion-molecule reaction products
The Chemical ionization mass spectra of free amino acids, their amides, methyl esters and Na-acetyl derivatgives, using methane as reagent gas, are presented, The occurrence and fragmentation ofExpand
Isobutane chemical ionization mass spectrometry of dipeptides.
The isobutane chemical ionization mass spectra of 25 dipeptides have been determined. All of the peptides gave spectra useful for identification and sequencing. The spectra of most peptides showedExpand
Comparative studies on photo‐ionization and electron‐impact ionization of peptide derivatives
Photo-ionization (PI) mass spectrometry performed with a monochromatic photon beam was applied to a series of peptide derivatives. PI mass spectra of ten N-acylpeptide methyl esters containing two toExpand
Advances in gas chromatographic mass spectrometric protein sequencing. 3—Automated interpretation of the mass spectra of the polyamino alcohol derivatives
A computer program, PEPALG, has been developed to interpret data generated in a gas chromatographic mass spectrometric experiment on a complex mixture of N-α, (ω)-trifluoroethyl-O-trimethylsilylExpand
The identification of depsipeptides by chemical ionisation mass spectroscopy.
The chemical ionisation mass spectra of seven naturally occurring depsipeptides and some of their permethylated derivatives have been measured and a mechanism for the primary fragmentation is proposed. Expand
Mass spectra of complex molecules. I. Chemical ionization of sphingolipids.
Methane chemical ionization spectra of acetylated and perdeutero-acetylated ceramides, cerebrosides, and ceramide dihexosides have been analyzed and compared with electron impact ionization Spectra of the same compounds to enable the determination of the molecular weights of the principal species of sphingolipid mixtures. Expand
Mass spectrometry is now established as a viable method for determing the sequence of amino acids in proteins and is especially useful in the detection and characterization of novel amino acids which might otherwise be difficult to identify. Expand
Acetone chemical ionization mass spectrometry
Chemical ionization mass spectrometry (CIMS) is often used for the characterization of thermally stable and electron impact-labile compounds, and acetone is one of the CI reagent gases used not onlyExpand
Analysis of biogenic amines and their metabolites by gas chromatography-chemical ionization mass spectrometry.
It was found that the ionization source temperature is an important factor in chemical ionization mass spectrometry, and suitable selection of the temperature is essential for obtaining good mass spectra. Expand


Detection and Location of N-Methyl-amino-acid Residues in N-Acyloligopeptide Methyl Esters by Mass Spectrometry
IN a previous communication1 we described a procedure for complete N-methylation of peptide –CONH– groups in oligopeptide derivatives. A mixture of methyl iodide and silver oxide in dimethylformamideExpand
Advantages and limitations of the mass spectrometric sequence determination of permethylated oligopeptide derivatives.
Abstract The mass spectrometric sequence determination of O,N-permethylated oligopeptide derivatives can be extremely facile because their spectra consist principally of peaks arising from CO-NCH 3Expand
N-methylation of N-acyl oligopeptides.
N-acyl oligopeptide methyl esters can be easily methylated to yield the permethylated derivatives, such as (V), which are more volatile than the non methylated compounds and can be used with advantage for the mass spectrometric determination of amino acid sequence. Expand
Massenspektrometrische Untersuchungen, IV. Massenspektren von N‐Acetyl‐peptiden einfacher Monoaminocarbonsäuren!
Die Massenspektren von 14 N-Acetyl-dipeptiden und N-Acetyl-tripeptiden sowie eines N-Acetyl-pentapeptids und eines cyclischen Hexapeptids werden angegeben. Die charakteristischen Ionen entstehenExpand
Sequenzanalyse durch Kombination von Gaschromatographie und Massenspektrometrie, II1) Die Struktur des Antamanids
Das antitoxische Cyclodecapeptid Antamanid aus dem grunen Knollenblatterpilz (Amanita phalloides) enthalt die Aminosauren Phe4Ala1Val1Pro4. Die kombinierte Anwendung von Gaschromatographie undExpand
Chemical ionization mass spectrometry of complex molecules. II. Alkaloids.
Chemical ionization mass spectrometry of complex molecules. IV. Amino acids.