Chemical ionization mass spectrometry of complex molecules—VI: Peptides†

@article{Kiryushkin1971ChemicalIM,
  title={Chemical ionization mass spectrometry of complex molecules—VI: Peptides†},
  author={A. Kiryushkin and H. Fales and T. Axenrod and E. J. Gilbert and G. Milne},
  journal={Journal of Mass Spectrometry},
  year={1971},
  volume={5},
  pages={19-31}
}
The chemical ionization mass spectra of a series of simple peptides containing six or fewer amino acids have been studied. Using methane as the reactant gas we found cleavage of the peptide bond occurs in two ways, yielding either the acyl carbonium ion or the complementary ammonium ion. The observation of both types of fragments permits the determination of the amino acid sequence of the peptide. The ammonium ions provide an additional sequence determining route compared to that available from… Expand
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References

SHOWING 1-10 OF 17 REFERENCES
Detection and Location of N-Methyl-amino-acid Residues in N-Acyloligopeptide Methyl Esters by Mass Spectrometry
IN a previous communication1 we described a procedure for complete N-methylation of peptide –CONH– groups in oligopeptide derivatives. A mixture of methyl iodide and silver oxide in dimethylformamideExpand
Advantages and limitations of the mass spectrometric sequence determination of permethylated oligopeptide derivatives.
Abstract The mass spectrometric sequence determination of O,N-permethylated oligopeptide derivatives can be extremely facile because their spectra consist principally of peaks arising from CO-NCH 3Expand
N-methylation of N-acyl oligopeptides.
TLDR
N-acyl oligopeptide methyl esters can be easily methylated to yield the permethylated derivatives, such as (V), which are more volatile than the non methylated compounds and can be used with advantage for the mass spectrometric determination of amino acid sequence. Expand
Massenspektrometrische Untersuchungen, IV. Massenspektren von N‐Acetyl‐peptiden einfacher Monoaminocarbonsäuren!
Die Massenspektren von 14 N-Acetyl-dipeptiden und N-Acetyl-tripeptiden sowie eines N-Acetyl-pentapeptids und eines cyclischen Hexapeptids werden angegeben. Die charakteristischen Ionen entstehenExpand
Sequenzanalyse durch Kombination von Gaschromatographie und Massenspektrometrie, II1) Die Struktur des Antamanids
Das antitoxische Cyclodecapeptid Antamanid aus dem grunen Knollenblatterpilz (Amanita phalloides) enthalt die Aminosauren Phe4Ala1Val1Pro4. Die kombinierte Anwendung von Gaschromatographie undExpand
Chemical ionization mass spectrometry of complex molecules. II. Alkaloids.
Chemical ionization mass spectrometry of complex molecules. IV. Amino acids.
...
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