Chemical genetics and cereal starch metabolism: structural basis of the non-covalent and covalent inhibition of barley β-amylase.

@article{Rejek2011ChemicalGA,
  title={Chemical genetics and cereal starch metabolism: structural basis of the non-covalent and covalent inhibition of barley $\beta$-amylase.},
  author={M. Rej{\vz}ek and C. Stevenson and A. Southard and D. Stanley and Kay Denyer and Alison M. Smith and M. Naldrett and D. Lawson and R. Field},
  journal={Molecular bioSystems},
  year={2011},
  volume={7 3},
  pages={
          718-30
        }
}
There are major issues regarding the proposed pathway for starch degradation in germinating cereal grain. Given the commercial importance but genetic intractability of the problem, we have embarked on a program of chemical genetics studies to identify and dissect the pathway and regulation of starch degradation in germinating barley grains. As a precursor to in vivo studies, here we report systematic analysis of the reversible and irreversible inhibition of the major β-amylase of the grain… Expand
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