Chemical extraction and direct adsorptive purification of recombinant human antigen Ro52.

Abstract

The antigenic protein Ro52 was expressed in the E. coli system harboring a 6 x His tag in the form of insoluble inclusion bodies. Direct chemical extraction of the product using 6-8 M urea proved to be effective. Furthermore, the tagged protein was recovered by direct adsorption on Ni2+-loaded commercial adsorbents derivatized with iminodiacetic acid… (More)

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@article{Saccodossi2004ChemicalEA, title={Chemical extraction and direct adsorptive purification of recombinant human antigen Ro52.}, author={Natalia Saccodossi and Rosa B Cabrera and Fernando Oliver and Lucila Gelmi and Daniel G Magglio and Hector M Fern{\'a}ndez-Lahore and Juliana Menegassi Leoni and Rita R Stumpo}, journal={Journal of separation science}, year={2004}, volume={27 7-8}, pages={589-94} }