Chemical de-O-glycosylation of glycoproteins for application in LC-based proteomics.


We describe a cyclic on-column procedure for the sequential degradation of complex O-glycans on proteins or peptides by periodate oxidation of sugars and cleavage of oxidation products by elimination. Desialylated glycoproteins were immobilized to alkali-stable, reversed-phase Poros 20 beads followed by two degradation cycles and the eluted apoproteins were… (More)
DOI: 10.1002/pmic.200800492

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