Chemical characterization of fluorescein isothiocyanate-protein conjugates.

@article{Jobbgy1966ChemicalCO,
  title={Chemical characterization of fluorescein isothiocyanate-protein conjugates.},
  author={Antal Jobb{\'a}gy and Katalin Kir{\'a}ly},
  journal={Biochimica et biophysica acta},
  year={1966},
  volume={124 1},
  pages={
          166-75
        }
}
CONJUGATION PARAMETERS DETERMINED WITH 14C‐FITC
TLDR
The present work was undertaken to assess the amount of fluorochrome present by tracing a radioactive atom instead of using absorptiometry in order to determine theamount of alteration of absorption of FITC on coupling to proteins and to compare fluoresceinprotein (F/P) ratios.
The properties of some 14 C-fluorescein-protein conjugates.
Conjugates of Immunoglobulin G with Different Fluorochromes. I. Characterization by Anionic‐exchange Chromatography
The conjugation of purified IgG for 1 hour at room temperature and pH 9.5 was found to be a simple and reproducible method useful with FITC. MRITC. RBITC and RB200SC fluorochromes. Anionic‐exchange
The effects of radioiodination and fluorescent labelling on albumin.
TLDR
It is concluded that the two fluorescent labels and possibly the radioiodine labelling method used here are unsuitable for certain studies of BSA, such as its adsorption at solid-liquid interfaces.
Fluorescence properties of free and protein bound fluorescein dyes. I. Macrospectrofluorometric measurements.
TLDR
Excitation and emission properties of fluorescein derivatives were studied macrofluorometrically and it is indicated that FDA gives about twice the fluorescence intensity compared to equal concentrations of FITC.
Evaluation of Fluorescein Isothiocyanate-labeled Whole Antiserum in the Immunofluorescent Identification of Microorganisms
TLDR
It was found that the prelabeled globulins were considerably less reactive than the postlabeled globeulins or the whole serum conjugates, and a larger amount of brightly staining reagent per milliliter of original serum could be obtained from labeled whole serum than from post labeled globulin.
Fluorescent Protein Conjugates
TLDR
The methods of characterization are based upon a variety of chemical, optical, immunological, and miscellaneous physical properties such as sedimentation constant, electrophoretic behavior, and solubility.
Studies on "nonspecific" binding.
TLDR
The binding of flourescein to bovine serum albumin was studied by equilibrium dialysis under various conditions and it was shown that even slight fragmentation caused a drastic decrease in the ability to bind fluorescein.
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References

SHOWING 1-10 OF 14 REFERENCES
The staining properties of human serum proteins conjugated with purified fluorescein isothiocyanate.
Summary Electrophoretic analysis has shown that six preparations of fluorescein isothiocyanate (FITC) from one source contain degradation products which exhibit essentially similar fluorescence
PURIFICATION AND ANALYSIS OF FLUORESCEIN-LABELED ANTISERA BY COLUMN CHROMATOGRAPHY.
Summary 1.Fluorescent rabbit anti-bovine serum albumin antibody with bright specific and negligible nonspecific staining was prepared from the conjugated antiserum by a single elution from a
Isothiocyanate compounds as fluorescent labeling agents for immune serum.
TLDR
The present report deals with the synthesis of stable, solid isothiocyanates of fluorescein and rhodamine B and the use of these two compounds in "labeling" antibodies for the specific staining of antigens.
FLUORESCENT LABELS FOR ANTIBODY PROTEINS. APPLICATION TO BACTERIAL IDENTIFICATION
TLDR
Of the different compounds available for labeling purposes, fluorescein (as the isocyanate derivative) has been most widely used, but this compound requires specialized chemical equipment for its preparation and is not available commercially, probably because of its relative instability.
A kinetic study on the labeling of serum globulin with fluorescein isothiocyanate by means of the gel filtration technique.
TLDR
It was found that Sephadex gel filtration could be used for the quantitative analysis of reactants by rapid separation and it was shown that the complex of papain and fluorescent antibody could be separated from free papain satisfactorily on a SepHadex G-75 column.
Staining of complement and modification of fluorescent antibody procedures.
TLDR
It has been shown in a variation of the indirect staining procedure that guinea pig complement can be used in the staining of several antigens in combination with antisera from various animal species.
Preparation and properties of fluorescent insulin derivatives.
Fluorescent treponemal antibody testing.
TLDR
The preparation of a fluorescein isothiocyanate immune serum conjugate is described, which is characterized by its antihuman-globulin titre, FITC/protein ratio and staining effect and a combination of the TPCF test with the cardiolipin complement-fixation test appears to be simpler, more sensitive and cheaper.
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