Chemical characterization of A beta 17-42 peptide, a component of diffuse amyloid deposits of Alzheimer disease.

@article{Gowing1994ChemicalCO,
  title={Chemical characterization of A beta 17-42 peptide, a component of diffuse amyloid deposits of Alzheimer disease.},
  author={E Gowing and Alex E. Roher and Amina S Woods and Robert J. Cotter and Matthew Chaney and Sheila P. Little and Melvyn J. Ball},
  journal={The Journal of biological chemistry},
  year={1994},
  volume={269 15},
  pages={10987-90}
}
A peptide corresponding to the amino acid sequence of A beta 17-42 (LVFFAEDVGSNKGAIIGLMVGGVVIA) was isolated from Alzheimer Disease patient brains containing large deposits of diffuse-type amyloid. Brain homogenates were lysed in SDS and submitted to high speed centrifugations. A beta peptides were purified by size exclusion chromatography on Superose 12 and TSK 3000 SW columns. An A beta peptide with M(r) of 3,000 was recovered that on automatic gas-phase Edman degradation yielded the amino… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 48 extracted citations

Similar Papers

Loading similar papers…