Chemical and thermal influence of the [4Fe-4S]2+ cluster of A/G-specific adenine glycosylase from Corynebacterium pseudotuberculosis.

@article{Eberle2015ChemicalAT,
  title={Chemical and thermal influence of the [4Fe-4S]2+ cluster of A/G-specific adenine glycosylase from Corynebacterium pseudotuberculosis.},
  author={Raphael Josef Eberle and M{\^o}nika Aparecida Coronado and {\'I}caro Putinhon Caruso and D{\'e}bora O Lopes and Anderson Miyoshi and Vasco Azevedo and Raghuvir Krishnaswamy Arni},
  journal={Biochimica et biophysica acta},
  year={2015},
  volume={1850 2},
  pages={393-400}
}
The gram-positive bacteria Corynebacterium pseudotuberculosis, the causative agent of caseous lymphadenitis in livestock significantly reduces productivity and often causes death. The adenine/guanine-specific DNA glycosylase (MutY) prevents mutations in the DNA of the pathogen and a unique feature of the MutY protein family is the [4Fe-4S]2+ cluster that interlinks two protein subdomains. MutY from C. pseudotuberculosis was expressed in E. coli and purified, the CD experiments indicate a high… CONTINUE READING