Chemical and structural characterization of a model Post-Termination Complex (PoTC) for the ribosome recycling reaction: Evidence for the release of the mRNA by RRF and EF-G

@inproceedings{Iwakura2017ChemicalAS,
  title={Chemical and structural characterization of a model Post-Termination Complex (PoTC) for the ribosome recycling reaction: Evidence for the release of the mRNA by RRF and EF-G},
  author={Nobuhiro Iwakura and Takeshi Yokoyama and Fabio Quaglia and Kaoru Mitsuoka and Kazuhiro Mio and Hideki Shigematsu and Mikako Shirouzu and Akira Kaji and H. Kaji},
  booktitle={PloS one},
  year={2017}
}
A model Post-Termination Complex (PoTC) used for the discovery of Ribosome Recycling Factor (RRF) was purified and characterized by cryo-electron microscopic analysis and biochemical methods. We established that the model PoTC has mostly one tRNA, at the P/E or P/P position, together with one mRNA. The structural studies were supported by the biochemical measurement of bound tRNA and mRNA. Using this substrate, we establish that the release of tRNA, release of mRNA and splitting of ribosomal… CONTINUE READING
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