Chemical activation of parthenogenetic and nuclear transfer porcine oocytes using ionomycin and strontium chloride.

@article{Che2007ChemicalAO,
  title={Chemical activation of parthenogenetic and nuclear transfer porcine oocytes using ionomycin and strontium chloride.},
  author={Limei Che and Arthur Lalonde and Vilceu Bordignon},
  journal={Theriogenology},
  year={2007},
  volume={67 7},
  pages={1297-304}
}
Effective protocols for oocyte activation are crucial for study of parthenogenetic development and to produce nuclear transfer reconstructed embryos. This study investigated the use of ionomycin (ION) and strontium chloride (Sr(2+)) in the activation of parthenogenetic and nuclear transfer porcine oocytes. In-vitro-matured oocytes with a polar body were treated with varying concentrations of ION, Sr(2+) or its combinations, and then fixed or cultured to assess activation and development rates… CONTINUE READING

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