Chemical Synthesis of Poliovirus cDNA: Generation of Infectious Virus in the Absence of Natural Template

@article{Cello2002ChemicalSO,
  title={Chemical Synthesis of Poliovirus cDNA: Generation of Infectious Virus in the Absence of Natural Template},
  author={Jerónimo Cello and Aniko Paul and Eckard Wimmer},
  journal={Science},
  year={2002},
  volume={297},
  pages={1016 - 1018}
}
Full-length poliovirus complementary DNA (cDNA) was synthesized by assembling oligonucleotides of plus and minus strand polarity. The synthetic poliovirus cDNA was transcribed by RNA polymerase into viral RNA, which translated and replicated in a cell-free extract, resulting in the de novo synthesis of infectious poliovirus. Experiments in tissue culture using neutralizing antibodies and CD155 receptor–specific antibodies and neurovirulence tests inCD155 transgenic mice confirmed that the… Expand
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References

SHOWING 1-10 OF 31 REFERENCES
Cell-free, de novo synthesis of poliovirus.
TLDR
Cell-free translation of poliovirus RNA in an extract of uninfected human (HeLa) cells yielded viral proteins through proteolysis of the polyprotein, which will aid in the study of picornavirus proliferation and in the search for the control of picORNaviral disease. Expand
Molecular cloning of poliovirus cDNA and determination of the complete nucleotide sequence of the viral genome.
  • V. Racaniello, D. Baltimore
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1981
TLDR
The complete 7410 nucleotide sequence of poliovirus type I genome was obtained from cloned cDNA was synthesized and inserted into the Pst I site of plasmid pBR322, and three clones were derived that together provided DNA copies of the entire poliov virus genome. Expand
Cellular receptor for poliovirus: Molecular cloning, nucleotide sequence, and expression of a new member of the immunoglobulin superfamily
TLDR
Northern hybridization analysis indicates that poliov virus receptor transcripts are expressed in a wide range of human tissues, in contrast to the limited expression of virus binding sites, which suggests that additional factors or modifications of the receptor protein are required to permit poliovirus attachment. Expand
Primary structure, gene organization and polypeptide expression of poliovirus RNA
TLDR
The primary structure of the poliovirus genome has been determined and Twelve viral polypeptides have been mapped by amino acid sequence analysis and were found to be proteolytic cleavage products of the polyprotein, cleavages occurring predominantly at Gln-Gly pairs. Expand
Genetic analysis of an NTP-binding motif in poliovirus polypeptide 2C.
TLDR
Viral RNA synthesis is greatly reduced but not entirely abolished in cells transfected with mutant RNAs, suggesting a functional role for the proposed NTP-binding motif of 2C in RNA replication and proliferation of poliovirus. Expand
The poliovirus receptor protein is produced both as membrane‐bound and secreted forms.
TLDR
Predicted amino acid sequences indicate that PVR alpha and PVR delta, corresponding to the previously described cDNA clones H20A and H20B, respectively, are integral membrane proteins while the other two molecules described here for the first time lack a putative transmembrane domain. Expand
Internal initiation of translation of eukaryotic mRNA directed by a sequence derived from poliovirus RNA
TLDR
A novel mechanism of initiation on poliovirus RNA occurs by binding of ribosomes to an internal sequence within the 5′ noncoding region, which may explain the disparate translation of several other eukaryotic messenger RNAs. Expand
A segment of the 5' nontranslated region of encephalomyocarditis virus RNA directs internal entry of ribosomes during in vitro translation
TLDR
The sequence between nucleotides 260 and 484 in the 5'NTR of encephalomyocarditis RNA was found to play a critical role in the efficient translation in both mono- and dicistronic mRNAs, and data suggest that an internal ribosomal entry site resides in this region. Expand
Production of a monoclonal antibody against an epitope on HeLa cells that is the functional poliovirus binding site.
TLDR
Results indicate that D171 recognizes the poliov virus receptor site on different susceptible cells and that practically all D171 binding sites are involved in the specific attachment of poliovirus to the plasma membrane. Expand
Transgenic mice susceptible to poliovirus.
  • S. Koike, C. Taya, +4 authors A. Nomoto
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1991
TLDR
The transgenic mice may become an excellent new animal model to study molecular mechanisms of pathogenesis of poliov virus and to assess oral poliovirus vaccines. Expand
...
1
2
3
4
...