Chemical Synthesis of Poliovirus cDNA: Generation of Infectious Virus in the Absence of Natural Template

@article{Cello2002ChemicalSO,
  title={Chemical Synthesis of Poliovirus cDNA: Generation of Infectious Virus in the Absence of Natural Template},
  author={Jerónimo Cello and Aniko Paul and Eckard Wimmer},
  journal={Science},
  year={2002},
  volume={297},
  pages={1016 - 1018}
}
Full-length poliovirus complementary DNA (cDNA) was synthesized by assembling oligonucleotides of plus and minus strand polarity. The synthetic poliovirus cDNA was transcribed by RNA polymerase into viral RNA, which translated and replicated in a cell-free extract, resulting in the de novo synthesis of infectious poliovirus. Experiments in tissue culture using neutralizing antibodies and CD155 receptor–specific antibodies and neurovirulence tests inCD155 transgenic mice confirmed that the… 
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878 Citations

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