Chemical Synthesis of Activity-Based Diubiquitin Probes.

Abstract

Activity-based diubiquitin probes are highly useful in probing the deubiquitinase (DUB) activity and ubiquitin chain linkage specificity. Here we describe a detailed protocol to synthesize a new class of diubiquitin DUB probes. In this method, two ubiquitin moieties are connected through a linker resembling the native linkage in size and containing a Michael acceptor for trapping the DUB active-site cysteine. Detailed procedures for generating the linker molecule are also described.

Cite this paper

@article{Li2017ChemicalSO, title={Chemical Synthesis of Activity-Based Diubiquitin Probes.}, author={Guorui Li and Libo Yuan and Zhihao Zhuang}, journal={Methods in molecular biology}, year={2017}, volume={1513}, pages={223-232} }