Chemical Protein Synthesis Using a Second-Generation N-Acylurea Linker for the Preparation of Peptide-Thioester Precursors.

@article{BlancoCanosa2015ChemicalPS,
  title={Chemical Protein Synthesis Using a Second-Generation N-Acylurea Linker for the Preparation of Peptide-Thioester Precursors.},
  author={Juan B. Blanco-Canosa and Brunello Nardone and Fernando Albericio and Philip E. Dawson},
  journal={Journal of the American Chemical Society},
  year={2015},
  volume={137 22},
  pages={7197-209}
}
The broad utility of native chemical ligation (NCL) in protein synthesis has fostered a search for methods that enable the efficient synthesis of C-terminal peptide-thioesters, key intermediates in NCL. We have developed an N-acylurea (Nbz) approach for the synthesis of thioester peptide precursors that efficiently undergo thiol exchange yielding thioester peptides and subsequently NCL reaction. However, the synthesis of some glycine-rich sequences revealed limitations, such as diacylated… CONTINUE READING
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