Chemical Modifications on Proteins Using Glutaraldehyde

  title={Chemical Modifications on Proteins Using Glutaraldehyde},
  author={Carla J. S. M. Silva and Fernanda Marinez de Sousa and Georg M. Guebitz and Artur Cavaco-Paulo},
In this work the effect of crosslinking the enzyme esperase (E.C. and the proteins bovine serum albumin and casein with the bifunctional compound glutaraldehyde on molecular mass increase was studied. Two common techniques for measuring molecular mass of proteins were used: SEC and SDS-PAGE. These techniques revealed that the proteins bovine albumin and casein, when subjected to chemical crosslinking with glutaraldehyde, volume fraction 0.25 %, increased their molecular mass by 20and… CONTINUE READING