Chemical-Inducible, Ecdysone Receptor-Based Gene Expression System for Plants

  title={Chemical-Inducible, Ecdysone Receptor-Based Gene Expression System for Plants},
  author={Malla Padidam and Michael Gore and D. Lily Lu and Olga Smirnova},
  journal={Transgenic Research},
We have developed an inducible gene expression system with potential for field application using the ecdysone receptor (EcR) from the spruce budworm and the non-steroidal EcR agonist, methoxyfenozide. Chimeric transcription activators were constructed with EcR ligand binding domain, GAL4 and LexA DNA binding domains, and VP16 activation domain. In the presence of methoxyfenozide, the transcription activators induced expression of the luciferase reporter gene cloned downstream of a promoter… CONTINUE READING

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