Chemical DNA sequencing in the opposite direction to the dideoxy chain termination method, using a single preparation of M13 single-stranded DNA.

Abstract

A strategy has been developed allowing the use of a single preparation of single-stranded DNA clones for chemical DNA sequencing in the opposite direction to the classical dideoxy chain termination method. Oligonucleotide complementary to the 5'-end of the multipurpose cloning sequence, with the proper restriction enzyme, is used to cleave specifically the molecules to expose a unique 5'-end, upstream to the inserted DNA, for the kinase labeling reaction. No further treatments are necessary before Maxam-Gilbert chemical sequencing reactions.

Cite this paper

@article{Bellemare1986ChemicalDS, title={Chemical DNA sequencing in the opposite direction to the dideoxy chain termination method, using a single preparation of M13 single-stranded DNA.}, author={Guy Bellemare and Claude L. Potvin and Claire Simard}, journal={Biochemistry and cell biology = Biochimie et biologie cellulaire}, year={1986}, volume={64 1}, pages={70-2} }