Checkpoints in Adenoviral Production: Cross-Contamination and E1A

@inproceedings{Hussler2011CheckpointsIA,
  title={Checkpoints in Adenoviral Production: Cross-Contamination and E1A},
  author={Dagmar Johanna Franziska H{\"a}ussler and Alicia M. Evangelista and Joseph Robert Burgoyne and Richard Alan Cohen and Markus M Bachschmid and David R. Pimental},
  booktitle={PloS one},
  year={2011}
}
Adenoviruses are widely used for overexpressing proteins in primary mammalian cells. Incorporation of the early viral gene, E1A, or viral cross-contamination can occur during amplification, and identification of these products is crucial as the transcription of unwanted genetic material can impact cell function and compromise data interpretation. Here we report methods for evaluation of contaminating adenovirus and E1 viral DNA. 
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