Charybdotoxin block of single Ca2+-activated K+ channels. Effects of channel gating, voltage, and ionic strength

@article{Anderson1988CharybdotoxinBO,
  title={Charybdotoxin block of single Ca2+-activated K+ channels. Effects of channel gating, voltage, and ionic strength},
  author={Carin S. Anderson and Richard MacKinnon and Curt Smith and Chester L. Miller},
  journal={The Journal of General Physiology},
  year={1988},
  volume={91},
  pages={317 - 333}
}
Charybdotoxin (CTX), a small, basic protein from scorpion venom, strongly inhibits the conduction of K ions through high-conductance, Ca2+-activated K+ channels. The interaction of CTX with Ca2+-activated K+ channels from rat skeletal muscle plasma membranes was studied by inserting single channels into uncharged planar phospholipid bilayers. CTX blocks K+ conduction by binding to the external side of the channel, with an apparent dissociation constant of approximately 10 nM at physiological… CONTINUE READING
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