Charge translocation coupled to electron injection into oxidized cytochrome c oxidase from Paracoccus denitrificans.

@article{Verkhovsky2001ChargeTC,
  title={Charge translocation coupled to electron injection into oxidized cytochrome c oxidase from Paracoccus denitrificans.},
  author={Michael I. Verkhovsky and Anne Tuukkanen and Camilla Ribacka n{\'e}e Backgren and Anne Puustinen and M{\aa}rten Wikstr{\"o}m},
  journal={Biochemistry},
  year={2001},
  volume={40 24},
  pages={7077-83}
}
Electrons were discretely injected into oxidized cytochrome c oxidase in liposomes by laser flash excitation of bound ruthenium [II] bispyridyl, and the membrane potential was recorded by time-resolved electrometry. Membrane potential is generated in a fast phase when an electron is transferred from the excited dye, via the CuA center, to heme a at a relative dielectric depth d inside the membrane [Zaslavsky, D., Kaulen, A. D., Smirnova, I. A., Vygodina, T., and Konstantinov, A. A. (1993) FEBS… CONTINUE READING
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