Charge reversal by salt-induced aggregation in aqueous lactoferrin solutions.

@article{Mela2010ChargeRB,
  title={Charge reversal by salt-induced aggregation in aqueous lactoferrin solutions.},
  author={Ioanna Mela and Elodie Aumaitre and Ann-Marie Williamson and Gleb E. Yakubov},
  journal={Colloids and surfaces. B, Biointerfaces},
  year={2010},
  volume={78 1},
  pages={53-60}
}
We have observed salt-induced aggregation in lactoferrin solutions using dynamic light scattering (DLS). Aggregates start to form once the ionic strength exceeds 10 mM, and are of opposite charge to their monomer building blocks. The presence of aggregates was monitored by electrophoretic measurements, in which the change of isoelectric point in lactoferrin solutions was observed and found to depend on the concentration of background electrolyte. Complimentary atomic force microscopy (AFM… CONTINUE READING