Characterizing transcription factor binding sites using formaldehyde crosslinking and immunoprecipitation.

@article{Wells2002CharacterizingTF,
  title={Characterizing transcription factor binding sites using formaldehyde crosslinking and immunoprecipitation.},
  author={Julie M. Wells and Peggy J. Farnham},
  journal={Methods},
  year={2002},
  volume={26 1},
  pages={
          48-56
        }
}
In their article, A.S. Weinmann and P.J. Farnham (2002, Methods 26) described new techniques for isolating in vivo binding sites for any DNA-binding protein. In this article, we describe complementary methods for detailed in vivo characterizations of such identified protein-DNA interactions. First, we describe how formaldehyde crosslinking and chromatin immunoprecipitation (ChIP), in conjunction with transient transfections or the use of cell lines containing stably integrated constructs or… CONTINUE READING
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