Characterizing the silica deposition vesicle of diatoms

  title={Characterizing the silica deposition vesicle of diatoms},
  author={Chao Li and S. Chu and Moo-Yeol Lee},
The present study on a centric diatom,Ditylum brightwellii, includes two parts: detection of sugars in the silica deposition vesicle (SDV) with lectins and labeling the developing siliceous cell wall in the SDV with rhodamine 123. Cells with developing valves are treated with SDS to remove all the cytoplasmic contents, then either stained with fluorescein labeled lectins or thin-sectioned and stained with colloidal gold labeled lectins. The results show that mannose is part of the organic… CONTINUE READING