Characterizing protein-glycosaminoglycan interactions using solution NMR spectroscopy.

  title={Characterizing protein-glycosaminoglycan interactions using solution NMR spectroscopy.},
  author={Prem Raj B Joseph and Krishna Mohan Poluri and Krishna Mohan Sepuru and Krishna Rajarathnam},
  journal={Methods in molecular biology},
Solution nuclear magnetic resonance (NMR) spectroscopy and, in particular, chemical shift perturbation (CSP) titration experiments are ideally suited for characterizing the binding interface of macromolecular complexes. (1)H-(15) N-HSQC-based CSP studies have become the method of choice due to their simplicity, short time requirements, and not requiring high-level NMR expertise. Nevertheless, CSP studies for characterizing protein-glycosaminoglycan (GAG) interactions have been challenging due… CONTINUE READING
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