Characterizing and optimizing protease/peptide inhibitor interactions, a new application for spot synthesis.

@article{Hilpert2000CharacterizingAO,
  title={Characterizing and optimizing protease/peptide inhibitor interactions, a new application for spot synthesis.},
  author={Kai Hilpert and Gerd Hansen and Helga Wessner and Jens Schneider-Mergener and Wolfgang H{\"o}hne},
  journal={Journal of biochemistry},
  year={2000},
  volume={128 6},
  pages={1051-7}
}
A new method is presented that uses parallel peptide array synthesis on cellulose membranes to characterize protease/peptide inhibitor interactions. A peptide comprising P5-P4' of the third domain of turkey ovomucoid inhibitor was investigated for both binding to and inhibition of porcine pancreatic elastase. Binding was studied directly on the cellulose membrane, while inhibition was measured by an assay in microtiter plates with punched out peptide spots. The importance of each residue for… CONTINUE READING

From This Paper

Topics from this paper.
10 Citations
0 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 10 extracted citations

Similar Papers

Loading similar papers…