Characterizing a thermostable Cas9 for bacterial genome editing and silencing

  title={Characterizing a thermostable Cas9 for bacterial genome editing and silencing},
  author={Ioannis Mougiakos and Prarthana Mohanraju and Elleke F. Bosma and Valentijn Vrouwe and Max Finger Bou and Mihris Ibnu Saleem Naduthodi and Alex Gussak and Rudolf B. L. Brinkman and Richard van Kranenburg and John van der Oost},
  booktitle={Nature Communications},
CRISPR-Cas9-based genome engineering tools have revolutionized fundamental research and biotechnological exploitation of both eukaryotes and prokaryotes. However, the mesophilic nature of the established Cas9 systems does not allow for applications that require enhanced stability, including engineering at elevated temperatures. Here we identify and characterize ThermoCas9 from the thermophilic bacterium Geobacillus thermodenitrificans T12. We show that in vitro ThermoCas9 is active between 20… CONTINUE READING
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