Characterization of zinc-depleted alanyl-tRNA synthetase from Escherichia coli: role of zinc.

@article{Sood1999CharacterizationOZ,
  title={Characterization of zinc-depleted alanyl-tRNA synthetase from Escherichia coli: role of zinc.},
  author={Satish M. Sood and Mei X. Wu and Kenneth A. Hill and Charles W. Slattery},
  journal={Archives of biochemistry and biophysics},
  year={1999},
  volume={368 2},
  pages={
          380-4
        }
}
To evaluate the role of zinc in Escherichia coli alanyl-tRNA synthetase, hydrodynamic measurements and circular dichroism spectra were obtained for the zinc-depleted protein and compared with those of the native enzyme. At a protein concentration of 5 mg ml(-1), pH 7.5, the sedimentation coefficient (s(20,w)) was 6.3 S and was virtually independent of temperature between 10 and 37 degrees C, similar to the results reported for the native form. However, the s(20,w) now decreased significantly as… CONTINUE READING

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