Characterization of wild-type human medium-chain acyl-CoA dehydrogenase (MCAD) and mutant enzymes present in MCAD-deficient patients by two-dimensional gel electrophoresis: evidence for post-translational modification of the enzyme.

@article{Bross1994CharacterizationOW,
  title={Characterization of wild-type human medium-chain acyl-CoA dehydrogenase (MCAD) and mutant enzymes present in MCAD-deficient patients by two-dimensional gel electrophoresis: evidence for post-translational modification of the enzyme.},
  author={Peter Alexander. Bross and Tina Gudrun Jensen and Brage S Andresen and Margrethe Kjeldsen and Andreas Nandy and Steen K{\o}lvraa and Sandro Ghisla and Ihab Rasched and Lars Bolund and N. Gregersen},
  journal={Biochemical medicine and metabolic biology},
  year={1994},
  volume={52 1},
  pages={
          36-44
        }
}
Two-dimensional gel electrophoresis was used to study and compare wild-type medium-chain acyl-CoA dehydrogenase (MCAD; EC 1.3.99.3) and mis-sense mutant enzyme found in patients with MCAD deficiency. By comparing the patterns for wild-type and mutant MCAD expressed in Escherichia coli or in eukaryotic COS-7 cells we demonstrate that variants with point mutations changing the net charge of the protein can be readily resolved from the wild-type protein. After expression of the cDNA in eukaryotic… CONTINUE READING

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